DiI-HDL Uptake Assay


Add DiI-HDL (final concentration 10μg/ml) to Dulbecco's Modified Eagle's Medium (CELLGRO, #10-013-CV)/Ham's F-12 medium (CELLGRO, #10-080-CV) (1:1), 50 units/ml penicillin, 50 µg/ml streptomycin, 2 mM glutamine (Invitrogen), 10% FBS (Fetal Bovine Serum, JRH Biosciences), 0.5% (w/v) BSA


Filter media through 0.22μm filter and add to cells (8-10 mls in 10-cm plate or 0.5 ml/well in 24-well plate


Incubate cells for 2 hrs at 37oC and observe with inverted fluorescence microscope

Wednesday, February 3, 2010

Molecular Biology, DNA, molecular biology, Molecular Biology Protocols, PCR, research, protocols, RNA, Protocols, biochemistry, cell biology, Laboratory, Current Protocols, procedures, laboratory protocols, Proteins, Biology, Cell Biology, CSH Protocols, molecular biology protocols
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